Lisa Jones, PhD, is an associate professor in the Department of Pharmaceutical Sciences at the University of Maryland School of Pharmacy.
About the Jones Lab:
The Jones Lab is a structural proteomics group that uses biochemical, analytical, and biophysical approaches to study protein interactions important in biological processes. Our research focuses on the use of protein foot printing methods coupled with mass spectrometry to identify these interactions. We are especially interested in the use of the footprinting method fast photochemical oxidation of proteins.
FPOP uses a pulsed excimer laser at 248 nm for photolysis of hydrogen peroxide of form hydroxyl radicals. These radicals them oxidize the side chains of amino acids. The power of the method lies in oxidatively modifying the samples in 2 states, such as ligand-bound and ligand-free. The modification differences between states provide information on ligand binding sites, protein interaction sites, and protein conformational change. We use FPOP to identify protein interactions and conformational change of various protein systems. We are especially interested in studying large, complex protein systems such as virus capsids and antibodies.
A major focus of the lab is extending FPOP as an in cell method for monitoring proteins in their native cellular environment. This method would be especially useful for membrane proteins, the largest class of drug targets, which are challenging to study in vitro owing to the difficulty of purifying these proteins. Currently, we are developing in cell FPOP using the chimeric protein GCamP2 as a model system.
The Jones Lab also utilizes protein footprinting methods coupled with mass spectrometry to characterize protein-protein interactions. We specifically use the hydroxyl radical footprinting method fast photochemical oxidation of proteins (FPOP) to study protein structure. In FPOP, hydroxyl radicals are generated by photolysis of hydrogen peroxide via an excimer laser.
For More Information:
Individuals and researchers who would like more information about the Jones Lab, or would like to collaborate with our team can contact:
Lisa Jones, PhD
Department of Pharmaceutical Sciences
University of Maryland School of Pharmacy
20 N. Pine Street, Room N719
Baltimore, MD 21201
Phone: (410) 706-3380